منابع مشابه
Lipase activation by nonionic detergents. The crystal structure of the porcine lipase-colipase-tetraethylene glycol monooctyl ether complex.
The crystal structure of the ternary porcine lipase-colipase-tetra ethylene glycol monooctyl ether (TGME) complex has been determined at 2.8 A resolution. The crystals belong to the cubic space group F23 with a = 289.1 A and display a strong pseudo-symmetry corresponding to a P23 lattice. Unexpectedly, the crystalline two-domain lipase is found in its open configuration. This indicates that in ...
متن کاملThe interaction of nonionic detergents with protein in paper electrophoresis.
Addition of polyoxyethylene nonionic detergents to the buffer in paper electrophoresis results in alteration of the rate of migration and shape of protein peaks. At low detergent concentrations, disruption of paper-protein binding leads to faster migration and sharper peaks. At high detergent concentrations, interaction of detergent with protein leadsto slower migration and broader peaks. On gl...
متن کاملReconstitution of Na+/K+-ATPase into phosphatidylcholine vesicles by dialysis of nonionic alkyl maltoside detergents.
The reconstitution of Na+/K+-ATPase from outer medulla of rabbit kidney into large unilamellar liposomes was achieved through detergent removal by dialysis of mixed micellar solutions of synthetic dioleoyl phosphatidylcholine/octyl glucoside and Na+/K+-ATPase/decyl maltoside or decenyl maltoside. Tight, transport-active liposomes were formed when the lipid and the enzyme were solubilized separa...
متن کاملElimination of Mycoplasma contaminants from virus stocks by treatment with nonionic detergents.
Five nonionic detergents (Tweens 20, 40, 60, and 80, and Triton WR-1339) were tested for their ability to inactivate four Mycoplasma species which are common contaminants of animal cell cultures. Tween 20 was found to be the most effective, in that a concentration of 2.5 mg/ml completely inactivated cultures of M. hominis, M. hyorhinis, and Acholeplasma laidlawii within 1 hr and a culture of M....
متن کاملEffect of nonionic detergents on amplification of human papillomavirus DNA with consensus primers MY09 and MY11.
Crude cell lysates, obtained by protein digestion and membrane lysis with nonionic detergents such as Nonidet P-40 (NP40), Tween 20, or Laureth 12, followed by heat denaturation, are suitable for PCR analysis (1). The L1 consensus PCR assay with the MY09-MY11 primer pair is widely utilized for detection of human papillomavirus (HPV) DNA (2, 3, 7, 10). However, the use of NP-40 is not proposed i...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1996
ISSN: 0021-9258
DOI: 10.1074/jbc.271.30.18007